qPCRBIO cDNA Synthesis Kit
High quality cDNA synthesis is essential for downstream real-time PCR analysis and successful expression studies. qPCRBIO cDNA Synthesis Kit is an easy-to-use 2 tube system specifically developed to generate cDNA for use in real-time PCR.
The reverse transcriptase, buffer system and optimised blend of random hexamers with anchored oligo(dT) primers provide unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations.
Features
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Unbiased representation of 5’ and 3’ mRNA transcript ends
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Sensitive detection of low copy number transcripts
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High cDNA yields from as little as 4pg total RNA
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Reduced RNase H activity
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Simple 2 tube system
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5x buffer contains anchored oligo(dT), random hexamers, enhancers, dNTPs and MgCl2
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20x thermostable reverse transcriptase blended with RNase inhibitor
Applications
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cDNA synthesis for real-time PCR analysis
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Low copy number transcripts
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Viral RNA targets
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Efficient synthesis from total RNA or poly(A)+ RNA