UltraScript 2.0 Reverse Transcriptase & Kits

UltraScript 2.0 Reverse Transcriptase & Kits

UltraScript 2.0 Reverse Transcriptase is a highly thermostable reverse transcriptase engineered for superior cDNA synthesis speed, yield and representation of the most challenging RNA sample types.
 
This modified MMLV reverse transcriptase can be used with reaction temperatures of over 55°C, giving improved specificity, higher cDNA yields and more full length cDNA product. 
 
Features
  • Highly thermostable reverse transcriptase 55°C to 65°C and above
  • Advanced RNase inhibitor
  • High cDNA yields from as little as 20pg total RNA
  • Accurate reverse transcription of GC-rich and highly structured transcripts
  • High sensitivity for challenging templates
  • Reduced RNase H activity
  • Available as a stand-alone enzyme with buffer, a cDNA synthesis kit with premixed oligos and a cDNA synthesis kit with separate oligos
Applications
  • cDNA synthesis for PCR analysis, cloning, cDNA library preparation and Next Generation Sequencing
  • Viral RNA targets
  • miRNA targets
  • Efficient synthesis from total RNA or poly(A)+ RNA