Supercritical Fluid Chromatography
Supercritical Fluid Chromatography
Innovative chiral separation technology used in Supercritical Fluid
The effect of the chiral compounds is the key factor in the pharmaceutical industry, and in order to evaluate the enantiomer, the chiral separation is taken up as a main theme. As a solution, Supercritical Fluid Chromatography (SFC) is drawing attention from many researchers.
Since the separation capacity of SFC is higher than the one of HPLC, the SFC is powerful tool for separating the chiral compounds which are hard to separate using HPLC.
SFC-4000 – Analytical systems
The physical characteristics exhibited by supercritical fluid include a diffusion coefficient of dissolved molecules that is a hundred times greater than it is in liquid and a viscosity that is at least one digit smaller. A SFC system, which employs such a medium as a mobile phase, can be expected to serve as a separation analysis method that can rapidly perform separation without any degradation in separation efficiency, even at fast flow rates, due to a rapid mass transfer inside the column when compared with high-speed liquid chromatography that uses liquid as the mobile phase.
SFC-4000 – Preparative systems
The Semi-Prep SFC and the Prep SFC system are applied to separation and purification with high recovery. When carbon dioxide is used as the medium, gasification will occur simply by keeping the separated and fractionated sample at an atmospheric pressure, making this one of the techniques capable of highly efficient refining with few post-processing hassles, such as elimination of solvents after preparative isolation. This offers a host of advantages, including cost cuts related to the expense of purchasing solvents and discarding organic solvents among other things.
Wide range of detectors
JASCO offers a wide range of detectors with high pressure cells UV, Diode Array (real time collection of 3D spectra and chromatogram) and the only CD detector available for SFC.
In particular, JASCO unique CD detector measures optical isomers with circular dichromatic absorption, and can measure both CD and UV chromatograms as well as g-factor (CD/UV) chromatograms. Since g-factor in particular has a proportional relationship with the compositional ratio of optical isomer samples, the CD detector can perform compositional measurements and high purity fractionation for non-separated peaks.
Method development by using SFC screening
Before separating and collecting the target chiral compound, finding the optimum separation condition is required (column, solvent, etc.). In order to create the measurement conditions and measure the samples automatically, JASCO can offer the SFC screening for method development, which has the benefits of laborsaving and operation improvement.
Improvement of sample throughput by stack injection
By shortening the sample injection interval to let chromatograms overlap, the separation in prep mode can be done efficiency. It means that the separation and purification of even a large amount of sample can be carried out with high recovery and high purity in short time.
Unique sample collection mechanism
In the SFC is using CO2 as the mobile phase, one of the main problems when collecting eluted sample is that the separated sample is blown apart due to volume expansion (approx. 500 times) of released CO2. In order to improve the collection rate, JASCO has developed a Micro Cyclone Separator (MCS) for Semi-Prep SFC system, and a dedicated fraction collector Prep SFC system.