PCRBIO HiFi Polymerase

PCRBIO HiFi Polymerase

PCRBIO HiFi Polymerase was derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity in PCR.

Several proprietary point mutations allow for significantly improved performance when compared with its native form. Together with advanced buffer chemistry this enzyme brings robust performance to the world of high fidelity PCR.

Features
  • 50x higher fidelity than Taq DNA polymerase
  • Increased PCR success rates with amplicons up to 10kb
  • Advanced buffer chemistry including Mg and dNTPs
  • High yields under standard and fast PCR conditions
  • Efficient and specific amplification from complex templates including GC rich and AT rich sequences
  • Stable at 25°C and 37°C for 4 weeks
Applications
  • High fidelity PCR
  • Blunt-end cloning
  • Site directed mutagenesis
  • Next generation sequencing
  • Crude sample PCR
  • Colony PCR
  • “Difficult” PCR - GC/AT rich DNA
  • Long range PCR - up to 10kb